A new technique for dechorionation and observations on the development of the naked egg in <i>Oryzias latipes</i>

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<jats:title>Abstract</jats:title><jats:p>A simple technique for removing the chorion (egg envelope) of <jats:italic>Oryzias latipes</jats:italic> eggs is described in which the chorion is mechanically cut off with microsurgical scissors before and immediately after insemination. When dechorionated eggs were inseminated, a high incidence of polyspermic fertilization was observed. Fusion was induced among dechorionated eggs that were in contact when they were inseminated with a high concentration of spermatozoa. However, monospermic eggs that were dechorionated immediately after completion of the cortical reaction induced by insemination changed shape periodically during cleavage and developed normally to fry.</jats:p>

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