Potential marker of oral squamous cell carcinoma aggressiveness detected by fluorescence in situ hybridization in fine‐needle aspiration biopsies

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<jats:title>Abstract</jats:title><jats:sec><jats:title>BACKGROUND</jats:title><jats:p>Amplification of chromosome 11q13 is a frequent event in carcinogenesis of the head and neck squamous cell carcinomas including oral carcinoma.</jats:p></jats:sec><jats:sec><jats:title>METHODS</jats:title><jats:p>Fluorescence in situ hybridization (FISH), using a BAC clone specific for the cyclin D1 gene (<jats:italic>CCND1</jats:italic>), was performed on specimens obtained by fine‐needle aspiration biopsy (FNAB) from 50 patients with primary oral squamous cell carcinomas (OSCCs.).</jats:p></jats:sec><jats:sec><jats:title>RESULTS</jats:title><jats:p>The <jats:italic>CCND1</jats:italic> numerical aberration was identified in 21 (42.0%) of 50 patients with primary OSCCs. The <jats:italic>CCND1</jats:italic> amplification was determined in 16 (32.0%) of these patients. Immunohistochemical staining revealed that all 21 tumors showing the <jats:italic>CCND1</jats:italic> numerical aberration overexpressed the <jats:italic>CCND1</jats:italic> protein. The <jats:italic>CCND1</jats:italic> numerical aberration was associated significantly with histopathologic grading (<jats:italic>P</jats:italic> = 0.032), the mode of invasion (<jats:italic>P</jats:italic> = 0.047), the presence of cancer cells at the resection margin (<jats:italic>P</jats:italic> = 0.033), pathologic lymph nodestatus (<jats:italic>P</jats:italic> = 0.045), disease recurrence (<jats:italic>P</jats:italic> = 0.004), and survival (<jats:italic>P</jats:italic> = 0.004). The disease‐free and overall survival period of patients with the <jats:italic>CCND1</jats:italic> numerical aberration was significantly shorter than that of patients without the <jats:italic>CCND1</jats:italic> numerical aberration (<jats:italic>P</jats:italic> = 0.0016 and <jats:italic>P</jats:italic> = 0.0019, respectively). Moreover, a multivariate analysis showed that the <jats:italic>CCND1</jats:italic> numerical aberration retained an independent prognostic value.</jats:p></jats:sec><jats:sec><jats:title>CONCLUSIONS</jats:title><jats:p>The <jats:italic>CCND1</jats:italic> numerical aberration is useful both as a prognostic indicator that is independent of the TNM classification, and an indicator to assist in determination of the appropriate treatment for patients with OSCCs. Analysis of the <jats:italic>CCND1</jats:italic> numerical aberration using FISH on FNABs may be a useful and practical method for predicting aggressive tumors, recurrence, and clinical outcome in patients with OSCCs. Cancer 2002;95:2152–9. © 2002 American Cancer Society.</jats:p><jats:p>DOI 10.1002/cncr.10929</jats:p></jats:sec>

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  • Cancer

    Cancer 95 (10), 2152-2159, 2002-10-31

    Wiley

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