Norepinephrine Facilitates Inhibitory Transmission in Substantia Gelatinosa of Adult Rat Spinal Cord (Part 2)

• Hiroshi Baba

  Lecturer, Department of Anesthesiology, Niigata University School of Medicine. Current position: Research Fellow, Department of Anesthesia, Neural Plasticity Research Group, Massachusetts General Hospital and Harvard Medical School, Charlestown, Massachusetts.

• Peter A. Goldstein

  Assistant Professor, Department of Anesthesiology, College of Physicians & Surgeons, Columbia University, New York, New York.

• Manabu Okamoto

  Lecturer, Department of Anesthesiology, Niigata University School of Medicine.

• Tatsuro Kohno

  Lecturer, Department of Anesthesiology, Niigata University School of Medicine.

• Toyofumi Ataka

  Graduate student, Department of Anesthesiology, Niigata University School of Medicine.

• Megumu Yoshimura

  Professor, Department of Physiology, Saga Medical School, Saga, Japan.

• Koki Shimoji

  Professor and Chairman, Department of Anesthesiology, Niigata University School of Medicine.

抄録

It has been reported previously that norepinephrine, when applied to the spinal cord dorsal horn, excites a subpopulation of dorsal horn neurons, presumably inhibitory interneurons. In the current study, the authors tested whether norepinephrine could activate inhibitory interneurons, specifically those that are "GABAergic." A transverse slice was obtained from a segment of the lumbar spinal cord isolated from adult male Sprague-Dawley rats. Whole-cell patch-clamp recordings were made from substantia gelatinosa neurons using the blind patch-clamp technique. The effects of norepinephrine on spontaneous GABAergic inhibitory postsynaptic currents were studied. In the majority of substantia gelatinosa neurons tested, norepinephrine (10-60 microM) significantly increased both the frequency and the amplitude of GABAergic inhibitory postsynaptic currents. These increases were blocked by tetrodotoxin (1 microM). The effects of norepinephrine were mimicked by the alpha1-receptor agonist phenylephrine (10-80 microM) and inhibited by the alpha1-receptor-antagonist WB-4101 (0.5 microM). Primary-afferent-evoked polysynaptic excitatory postsynaptic potentials or excitatory postsynaptic currents in wide-dynamic-range neurons of the deep dorsal horn were also attenuated by phenylephrine (40 microM). The observations suggest that GABAergic interneurons possess somatodendritic alpha1 receptors, and activation of these receptors excites inhibitory interneurons. The alpha1 actions reported herein may contribute to the analgesic action of intrathecally administered phenylephrine.

収録刊行物

• Anesthesiology

  Anesthesiology 92 (2), 485-485, 2000-02-01

  Ovid Technologies (Wolters Kluwer Health)

キーワード

• Anesthesiology and Pain Medicine

詳細情報

• CRID: 1363670320536427776
• NII論文ID: 30009126137
• DOI: 10.1097/00000542-200002000-00031
• ISSN: 00033022
• Web Site: http://pubs.asahq.org/anesthesiology/article-pdf/92/2/485/403830/0000542-200002000-00031.pdf
• Web Site: http://journals.lww.com/00000542-200002000-00031
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