Fourteen Anonymous DNA Markers of Laboratory Rats Identified with Arbitrarily Primed Polymerase Chain Reaction (AP-PCR).

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  • KURAMOTO Takashi
    Institute of Laboratory Animals, Faculty of Medicine, Kyoto University
  • MASHIMO Tomoji
    Institute of Laboratory Animals, Faculty of Medicine, Kyoto University
  • SERIKAWA Tadao
    Institute of Laboratory Animals, Faculty of Medicine, Kyoto University

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タイトル別名
  • Fourteen Anonymous DNA Markers of Labor

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抄録

In order to develop genetic markers in rats, arbitrarily primed polymerase chain reaction (AP-PCR) was performed with single primers originally designed for microsatellite loci, instead of primers with short-sized and arbitrary nucleotide sequences. Each primer generated reliable and reproducible segments under optimal conditions. The fourteen amplification products have been successfully mapped to rat chromosomes by either linkage analysis using backcross progeny or chromosomal assignment with somatic cell hybrids. All of the loci were located on different chromosomes from those of the microsatellite loci, suggesting that sequence-specifically designed single primers can produce anonymous segments of genomic DNA showing polymorphisms. These markers should contribute to finding linkages for traits of interest in rats.<br>

収録刊行物

  • Experimental Animals

    Experimental Animals 44 (2), 119-125, 1995

    公益社団法人 日本実験動物学会

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