Anti‐obesity action of <i>Salix matsudana</i> leaves (Part 2). Isolation of anti‐obesity effectors from polyphenol fractions of <i>Salix matsudana</i>
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<jats:title>Abstract</jats:title><jats:p>Previously, it was reported that polyphenol fractions prepared from the leaves of <jats:italic>Salix matsudana</jats:italic> reduced the elevation of the rat plasma triacylglycerol level at 3 and 4 h after oral administration of a lipid emulsion containing corn oil, at a dose of 570 mg/kg. Moreover, body weights at 2–9 weeks and the final parametrial adipose tissue weights were significantly lower in mice fed the high‐fat diet with 5% polyphenol fractions of <jats:italic>S. matsudana</jats:italic> leaves than in those fed the high‐fat diet alone. The polyphenol fractions of <jats:italic>S. matsudana</jats:italic> leaves also significantly reduced the hepatic total cholesterol content, which was elevated in mice fed the high‐fat diet alone. In addition, the polyphenol fractions of <jats:italic>S. matsudana</jats:italic> leaves inhibited palmitic acid uptake into brush border membrane vesicles prepared from rat jejunum and <jats:italic>α</jats:italic>‐amylase activity, and their fractions enhanced norepinephrine‐induced lipolysis in fat cells. To clarify the active substances inhibiting the palmitic acid uptake into small intestinal brush border membrane, the <jats:italic>α</jats:italic>‐amylase activity or enhancing the norepinephrine‐induced lipolyis in fat cells, the isolation of the active substances from polyphenol fraction was attempted using the above three assay systems. Compounds 1, 2 and 3 were isolated from the polyphenol fractions and identified as apigenin‐7‐<jats:italic>O</jats:italic>‐<jats:sc>d</jats:sc>‐glucoside, luteolin‐7‐<jats:italic>O</jats:italic>‐<jats:sc>d</jats:sc>‐glucoside and chrysoeriol‐7‐<jats:italic>O</jats:italic>‐<jats:sc>d</jats:sc>‐glucoside, respectively. Among three flavonoids, apigenin‐7‐<jats:italic>O</jats:italic>‐<jats:sc>d</jats:sc>‐glucoside inhibited <jats:italic>α</jats:italic>‐amylase activity, and luteolin‐7‐<jats:italic>O</jats:italic>‐<jats:sc>d</jats:sc>‐glucoside and chrysoeriol‐7‐<jats:italic>O</jats:italic>‐<jats:sc>d</jats:sc>‐glucoside inhibited palmitic acid uptake into small intestinal brush border membrane. Furthermore, three flavonoid glucosides enhanced norepinephrine‐induced lipolysis in fat cells. Copyright © 2003 John Wiley & Sons, Ltd.</jats:p>
収録刊行物
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- Phytotherapy Research
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Phytotherapy Research 17 (10), 1195-1198, 2003-12
Wiley
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詳細情報 詳細情報について
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- CRID
- 1360855570776787072
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- NII論文ID
- 30011908055
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- DOI
- 10.1002/ptr.1405
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- ISSN
- 10991573
- 0951418X
- http://id.crossref.org/issn/0951418X
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