Isologous monoclonal antibodies can induce anti‐GBM glomerulonephritis in rats

DOI Web Site Web Site 被引用文献10件

抄録

<jats:title>Abstract</jats:title><jats:p>Injection of isologous monoclonal antibodies (SR2, SR3) caused anti‐glomerular basement membrane antibody‐induced glomerulonephritis (anti‐GBM nephritis) in WKY/NCrj rats. The antibodies were obtained from hybridoma cells derived from fusion of the spleen of a nephritic WKY/NCrj ral injected with rat solubilized renal basement membranes with adjuvant, and mouse SP2 myeloma cells. They belonged to the rat IgG2a subclass and bound to rat kidney in a linear pattern along the glomerular and tubular basement membranes. Histological changes in glomeruli were detected at day 1 after the injection; proteinuria with haematuria appeared on day 2; and proteinuria became severe and reached a plateau by day 5. These results demonstrate that anti‐GBM nephritis can even be induced by an isologous monoclonal antibody and that the rat IgG2a subclass is at least nephritogenic. The experimental model of anti‐GBM nephritis with isologous monoclonal antibodies makes it possible and easier to analyse further the mechanism of anti‐GBM nephritis.</jats:p>

収録刊行物

被引用文献 (10)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ