Behavior of human periodontal ligament cells on CO₂ laser irradiated dentinal root surfaces: an <i>in vitro</i> study

• V. Pant

• J. Dixit

• A. K. Agrawal

• P. K. Seth

• A. B. Pant

抄録

Objective:  The aim of this study was to investigate the in vitro attachment behavior of human periodontal ligament fibroblasts on periodontally involved root surface after conditioning with CO 2 laser and to compare its efficacy with chemical conditioning agents, namely tetracycline hydrochloride, citric acid, hydrogen peroxide (H 2 O 2 ) and EDTA, using scanning electron microscopy. Methods:  A total of 84 scaled and root‐planed specimens from periodontally involved single‐rooted human teeth showing hopeless prognosis were selected and assigned to two groups. One group was lased with a CO 2 laser (from 5 cm at 3 W for 0.8, 1.0 and 1.2 s), and the other group was treated with either tetracycline hydrochloride (2.5%), citric acid (saturated solution, pH 1), H 2 O 2 (6%) or EDTA (5%; pH 7.4) for 3 min. The specimens were then seeded with human periodontal ligament fibroblasts, incubated for either 12 h or 24 h, and then the cell attachment behavior was observed. Results:  CO 2 laser irradiation for 1.0 s was found to be the most efficient, showing consistently good cell attachment with the highest mean value (15.00 ± 3.41 cells/10,000 µm 2 after incubation for 12 h and 29.17 ± 2.04 cells/10,000 µm 2 after 24 h), followed by irradiation for 0.8 s (13.11 ± 3.04 cells/10,000 µm 2 after incubation for 12 h and 22.91 ± 7.10 cells/10,000 µm 2 after 24 h). Charring was observed following irradiation for 1.2 s. Amongst chemical conditioning agents, citric acid was found to be the most efficient, with a mean cell attachment of 17.82 ± 2.16 cells/10,000 µm 2 after incubation for 12 h and 23.62 ± 1.94 cells/10,000 µm 2 after 24 h. EDTA and H 2 O 2 did not do well in the study. Conclusion:  The results suggest that CO 2 laser irradiation for 1.0 s may promote comparatively better attachment of periodontal ligament fibroblast on dentinal root surfaces than the conventional chemical conditioning agents used in the study.

収録刊行物

• Journal of Periodontal Research

  Journal of Periodontal Research 39 (6), 373-379, 2004-08-24

  Wiley

キーワード

• Periodontics

詳細情報

• CRID: 1360011143548747264
• NII論文ID: 30013419489
• DOI: 10.1111/j.1600-0765.2004.00751.x
• ISSN: 16000765
• ISSN: 00223484
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1111%2Fj.1600-0765.2004.00751.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1111/j.1600-0765.2004.00751.x
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