The Mechanism of Enzymatic Cellulose Degradation

DOI Web Site Web Site 被引用文献4件

書誌事項

タイトル別名
  • Purification of a Cellulolytic Enzyme from <i>Trichoderma viride</i> Active on Highly Ordered Cellulose

抄録

<jats:p> <jats:list list-type="explicit-label"> <jats:list-item><jats:p>A cellulolytic enzyme (“C<jats:sub>1</jats:sub>” enzyme) has been isolated from a commercial cellulase preparation derived from culture filtrates of the fungus <jats:italic>Trichoderma viride</jats:italic>.</jats:p></jats:list-item> <jats:list-item><jats:p>The purification method is a four‐step procedure including chromatography on Bio‐Gel P‐10, DEAE‐Sephadex chromatography, isoelectric focusing and chromatography on Bio‐Gel P‐60.</jats:p></jats:list-item> <jats:list-item><jats:p>A yield of 144 mg enzyme was obtained per 100 g commercial cellulase.</jats:p></jats:list-item> <jats:list-item><jats:p>The isolated enzyme was homogeneous in polyacrylamide gel electrophoresis at pH 5.0 and at pH 8.0 by isoelectric focusing in a polyacrylamide gel and also in the ultracentrifuge.</jats:p></jats:list-item> <jats:list-item><jats:p>No enzyme activity towards carboxymethylcellulose could be detected in the purified material under the assay conditions used. Similarly, there was no β‐glucosidase activity.</jats:p></jats:list-item> <jats:list-item><jats:p>The purified enzyme was associated with 3.3% carbohydrate and is assumed to be a glycoprotein. The enzyme was isoelectric at pH 3.79 (10°C). A molecular weight of 46000 was determined by chromatography of the reduced and alkylated enzyme on a calibrated column of Sepharose 6B in 6 M guanidine‐HCl.</jats:p></jats:list-item> <jats:list-item><jats:p>Crystalline cellulose (Avicel), phosphoric acid‐swollen Avicel and cellotetraose were degraded by the enzyme and in each case the principle reaction product was cellobiose.</jats:p></jats:list-item> <jats:list-item><jats:p>Evidence indicates that the purified enzyme is a β‐1,4‐glucan cellobiohydrolase.</jats:p></jats:list-item> </jats:list> </jats:p>

収録刊行物

被引用文献 (4)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ