Opioid withdrawal activates MAP kinase in locus coeruleus neurons in morphine‐dependent rats <i>in vivo</i>

DOI Web Site Web Site 被引用文献1件

この論文をさがす

抄録

<jats:title>Abstract</jats:title><jats:p>Opioid dependence is widely believed to result from neuroadaptations in specific brain regions. However, the precise molecular mechanisms underlying these adaptations are not yet clear. Our aim was to explore the role of mitogen‐activated protein kinase (MAPK) in μ opioid receptor signalling <jats:italic>in vivo</jats:italic>. Using anti‐phospho MAPK antibodies, activated MAPK was detected in cortical neurons (layers II/III), median eminence, amygdaloid and hypothalamic nuclei in untreated animals. Dense nuclear and cytoplasmic staining was observed resulting in full visualization of processes in these cells. Chronic, but not acute, administration of morphine greatly diminished this staining pattern while μ opioid receptor levels and levels of MAPkinase as detected with a phosphorylation state‐independent antibody were unchanged. When opioid withdrawal was precipitated with naloxone a dramatic increase in MAPkinase phosphorylation was observed in somata and fibres of locus coeruleus, solitary tract and hypothalamic neurons. Thus, the differential activation state of MAPK could have important implications for understanding the mechanisms underlying opioid tolerance and dependence.</jats:p>

収録刊行物

被引用文献 (1)*注記

もっと見る

キーワード

詳細情報 詳細情報について

問題の指摘

ページトップへ