GABA<sub>B</sub>‐receptor splice variants GB1a and GB1b in rat brain: developmental regulation, cellular distribution and extrasynaptic localization
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<jats:title>Abstract</jats:title><jats:p>GABA<jats:sub>B</jats:sub> (γ‐aminobutyric acid)‐receptors have been implicated in central nervous system (CNS) functions, e.g. cognition and pain perception, and dysfunctions including spasticity and absence epilepsy. To permit an analysis of the two known GABA<jats:sub>B</jats:sub>‐receptor splice variants GABA<jats:sub>B</jats:sub>‐R1a (GB1a) and GABA<jats:sub>B</jats:sub>‐R1b (GB1b), their distribution pattern has been differentiated in the rat brain, using Western blotting and immunohistochemistry with isoform‐specific antisera. During postnatal maturation, the expression of the two splice variants was differentially regulated with GB1a being preponderant at birth. In adult brain, GB1b‐immunoreactivity (‐IR) was predominant, and the two isoforms largely accounted for the pattern of GABA<jats:sub>B</jats:sub>‐receptor binding sites in the brain. Receptor heterogeneity was pronounced in the hippocampus, where both isoforms occurred in CA1, but only GB1b in CA3. Similarly, in the cerebellum, GB1b was exclusively found in Purkinje cells in a zebrin‐like pattern. The staining was most pronounced in Purkinje cell dendrites and spines. Using electron microscopy, over 80% of the spine profiles in which a synaptic contact with a parallel fibre was visible contained GB1b‐IR at extrasynaptic sites. This subcellular localization is unrelated to GABAergic inputs, indicating that the role of GABA<jats:sub>B</jats:sub>‐receptors <jats:italic>in vivo</jats:italic> extends beyond synaptic GABAergic neurotransmission and may, in the cerebellum, involve taurine as a ligand.</jats:p>
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- European Journal of Neuroscience
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European Journal of Neuroscience 11 (3), 761-768, 1999-03
Wiley
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詳細情報 詳細情報について
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- CRID
- 1360011145608145280
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- NII論文ID
- 30014382461
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- NII書誌ID
- AA10672270
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- ISSN
- 14609568
- 0953816X
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- データソース種別
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