Hybrid Artificial Liver Using Hepatocyte Organoid Culture

• Kazumori Funatsu

• Hiroyuki Ijima

• Kohji Nakazawa

• Yo‐ichi Yamashita

• Mitsuo Shimada

• Keizo Sugimachi

抄録

Abstract: We developed 2 types of hybrid artificial liver modules using hepatocyte organoid culture. One was a polyurethane foam (PUF)/hepatocyte spheroid packed‐bed module. Hepatocytes spontaneously formed spheroids in the PUF pores, and they maintained liver‐specific functions well for at least 2 weeks in vitro. As a preclinical experiment, a hybrid artificial liver with 200 g porcine hepatocytes was applied to a pig (25 kg) with liver failure and showed that the hybrid artificial liver was effective in support of liver functions and stabilization of general conditions. We established a new technique of hepatocyte organoid formation using centrifugal force. A hepatocyte organoid formed by centrifugation in hollow fibers maintained functions for more than 4 months in vitro. We developed a new sinusoid‐like structure module having hollow fibers arranged by spacers in a micro‐regular arrangement. Inoculated hepatocytes in the extra‐fiber space of the module formed the organoid by centrifugation, and they maintained the functions for at least 1 month in vitro. The results indicated that this module seems to be promising as a hybrid artificial liver.

収録刊行物

• Artificial Organs

  Artificial Organs 25 (3), 194-200, 2001-03

  Wiley

キーワード

• Biomedical Engineering
• General Medicine
• Biomaterials
• Medicine (miscellaneous)
• Bioengineering

詳細情報

• CRID: 1361981469401315456
• NII論文ID: 30014488831
• DOI: 10.1046/j.1525-1594.2001.025003194.x
• ISSN: 15251594
• ISSN: 0160564X
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1046%2Fj.1525-1594.2001.025003194.x
• Web Site: https://onlinelibrary.wiley.com/doi/pdf/10.1046/j.1525-1594.2001.025003194.x
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