Two classes of <i>cis</i> sequences contribute to tissue‐specific expression of a <i>PAL2</i> promoter in transgenic tobacco

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<jats:p>Genes encoding phenylalanine ammonia‐lyase (PAL) are expressed in a complex pattern during plant development and in response to light, pathogen ingress, mechanical damage and other stresses. Analysis of the promoter of the bean <jats:italic>PAL2</jats:italic> gene in transgenic tobacco has shown that some regions responsible for developmental expression are functionally compensatory. The minimum sequence containing all <jats:italic>cis</jats:italic> sequences necessary for developmental patterns of expression is within −254 bp of the transcription start site. Footprinting and electrophoretic mobility shift assay studies of this region revealed potential <jats:italic>cis</jats:italic> sequences which coincided with the functional domains defined by small deletions and promoter fusions. Mutations in these potential <jats:italic>cis</jats:italic> sequences in the context of the minimal −254 bp promoter altered tissue‐specific expression patterns, confirming the importance of these sequences for expression <jats:italic>in vivo</jats:italic>. A functional model for the promoter is presented which predicts that three AC‐elements, which are possible Myb protein binding sites, together with a G‐box, interact to direct the complex patterns of tissue‐specific expression observed.</jats:p>

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