Characterization of the <i>Drosophila Methoprene</i> ‐tolerant gene product
書誌事項
- タイトル別名
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- Juvenile hormone binding and ligand‐dependent gene regulation
抄録
<jats:p>Juvenile hormones (JHs) of insects are sesquiterpenoids that regulate a great diversity of processes in development and reproduction. As yet the molecular modes of action of JH are poorly understood. The <jats:italic>Methoprene‐tolerant</jats:italic> (<jats:italic>Met</jats:italic>) gene of <jats:italic>Drosophila melanogaster</jats:italic> has been found to be responsible for resistance to a JH analogue (JHA) insecticide, methoprene. Previous studies on <jats:italic>Met</jats:italic> have implicated its involvement in JH signaling, although direct evidence is lacking. We have now examined the product of <jats:italic>Met</jats:italic> (MET) in terms of its binding to JH and ligand‐dependent gene regulation. <jats:italic>In vitro</jats:italic> synthesized MET directly bound to JH III with high affinity (<jats:italic>K</jats:italic><jats:sub>d</jats:sub> = 5.3 ± 1.5 n<jats:sc>m</jats:sc>, mean ± SD), consistent with the physiological JH concentration. In transient transfection assays using <jats:italic>Drosophila</jats:italic> S2 cells the yeast GAL4‐DNA binding domain fused to MET exerted JH‐ or JHA‐dependent activation of a reporter gene. Activation of the reporter gene was highly JH‐ or JHA‐specific with the order of effectiveness: JH III ≫ JH II > JH I > methoprene; compounds which are only structurally related to JH or JHA did not induce any activation. Localization of MET in the S2 cells was nuclear irrespective of the presence or absence of JH. These results suggest that MET may function as a JH‐dependent transcription factor.</jats:p>
収録刊行物
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- The FEBS Journal
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The FEBS Journal 272 (5), 1169-1178, 2005-02-17
Wiley
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詳細情報 詳細情報について
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- CRID
- 1361699994248849536
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- NII論文ID
- 30014772456
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- ISSN
- 17424658
- 1742464X
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- データソース種別
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