Ethanol intake preceding <i>Helicobacter pylori</i> inoculation promotes gastric mucosal inflammation in Mongolian gerbils

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<jats:title>Abstract</jats:title><jats:p> <jats:bold> <jats:italic>Background</jats:italic> </jats:bold>: Mongolian gerbils have been reported to be a suitable model for <jats:italic>Helicobacter pylori</jats:italic>‐associated gastric mucosal injury, including gastric cancer. Although ethanol is known to be one of the harmful substances in the gastric mucosa, the relationship between ethanol and <jats:italic>H. pylori</jats:italic> infection remains unknown. The aim of the present study is to investigate the effect of ethanol treatment prior to <jats:italic>H. pylori</jats:italic> inoculation on associated gastric mucosal injury.</jats:p><jats:p> <jats:bold> <jats:italic>Methods</jats:italic> </jats:bold>: Male Mongolian gerbils were used for the study. <jats:italic>Helicobacter pylori</jats:italic> was orally inoculated after 15 h fasting (Hp group). Thirty minutes prior to <jats:italic>H. pylori</jats:italic> inoculation, a group of gerbils was orally treated with 40% ethanol (20 mL/kg; E + Hp group). Another group of animals was treated either with <jats:italic>H. pylori</jats:italic> culture media alone (controls) or with 40% ethanol plus culture media (E group). Gerbils were killed 2, 4 or 12 weeks after <jats:italic>H. pylori</jats:italic> inoculation. <jats:italic>Helicobacter pylori</jats:italic> infection was confirmed by both histological examination and serological tests. Mucosal damage was evaluated histologically according to the modified Sydney system.</jats:p><jats:p> <jats:bold> <jats:italic>Results</jats:italic> </jats:bold>: Although in the controls and E group no significant change to the gastric mucose was observed, persistent <jats:italic>H. pylori</jats:italic> infection was seen in the mucosa and mucosal leucocyte infiltration and severe epithelial damage was observed in the Hp and E + Hp groups after 4 weeks. The histological scores for polymorphonuclear cell infiltration and myeloperoxidase activity were higher in the E + Hp group at 4 weeks than in the Hp group (<jats:italic>P</jats:italic> < 0.05).</jats:p><jats:p> <jats:bold> <jats:italic>Conclusions</jats:italic> </jats:bold>: Ethanol intake preceding <jats:italic>H. pylori</jats:italic> inoculation could promote the progression of gastric mucosal inflammation in Mongolian gerbils.</jats:p><jats:p>© 1999 Blackwell Science Asia Pty Ltd</jats:p>

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