Conjugated α‐linolenic acid production from α‐linolenic acid by <i>Lactobacillus plantarum</i> AKU 1009a

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<jats:title>Abstract</jats:title><jats:p>Conjugated α‐linolenic acid (CALA) was produced by incubation of α‐linolenic acid with the washed cells of <jats:italic>Lactobacillus plantarum</jats:italic> AKU 1009a. The washed cells expressing high levels of CALA productivity were obtained by cultivation in a nutrient medium supplemented with 0.01% (wt/vol) α‐linolenic acid as an inducer. Under the optimum reaction conditions with 63 mg/ml α‐linolenic acid as substrate, the washed cells produced 25 mg CALA/ml reaction mixture (40 mol‐% yield) in 72 h. The produced CALA were a mixture of the two isomers, <jats:italic>i.e</jats:italic>., <jats:italic>cis</jats:italic>‐9, <jats:italic>trans</jats:italic>‐11, <jats:italic>cis</jats:italic>‐15‐octadecatrienoic acid (CALA1, 67% of total CALA) and <jats:italic>trans</jats:italic>‐9, <jats:italic>trans</jats:italic>‐11, <jats:italic>cis</jats:italic>‐15‐octadecatrienoic acid (CALA2, 33% of total CALA), and accounted for 48% of total fatty acid obtained. Almost stoichiometric conversion was attained with 12 mg/ml α‐linolenic acid as the substrate in 48 h reaction time. It resulted in 12 mg CALA/ml reaction mixture consisting of 43% CALA1 and 57% CALA2 accounting for 66% of total fatty acid obtained. The CALA produced was obtained as the free fatty acid.</jats:p>

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