Fluorescence decay and depolarization in membranes

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  • D. Toptygin
    McCollum-Pratt Institute, The Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218
  • J. Svobodova
    McCollum-Pratt Institute, The Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218
  • I. Konopasek
    McCollum-Pratt Institute, The Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218
  • L. Brand
    McCollum-Pratt Institute, The Department of Biology, The Johns Hopkins University, Baltimore, Maryland 21218

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<jats:p>In optically discontinuous and/or anisotropic environments such as lipid membranes, the radiative decay rate is dependent on the orientation of the emission dipole. A theory of fluorescence decay and depolarization in membranes has been developed that takes the orientational dependence into account. The theory predicts nonexponential total emission intensity decay and emission anisotropy decay different than the one in optically isotropic systems. 1,6-diphenyl-1,3,5-hexatriene fluorescence in L-α-dipalmitoyl-phosphatidylcholine large unilamellar vesicles has been analyzed in terms of this theory. It has been shown that the orientational dependence of the radiative rate is sufficient to account for the complexity of the intensity decay in membranes. The analysis also allowed the determination of the refractive index, orientational order, and rotational dynamics in the membrane.</jats:p>

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