Fluorescence Activated Cell Sorting

• W. A. Bonner

  Department of Genetics, Stanford University School of Medicine, Stanford, California 94305

• H. R. Hulett

  Department of Genetics, Stanford University School of Medicine, Stanford, California 94305

• R. G. Sweet

  Department of Genetics, Stanford University School of Medicine, Stanford, California 94305

• L. A. Herzenberg

  Department of Genetics, Stanford University School of Medicine, Stanford, California 94305

Abstract

An instrument has been developed for sorting biological cells. The cells are rendered differentially fluorescent and incorporated into a small liquid stream illuminated by a laser beam. The cells pass sequentially through the beam, and fluorescent light from the cells gives rise to electrical signals. The stream is broken into a series of uniform size drops downstream of the laser. The cell signals are used to give appropriate electrostatic charges to drops containing the cells. The drops then pass between two charged plates and are deflected to appropriate containers. The system has proved capable of providing fractions containing large numbers of viable cells highly enriched in a particular functional type.

Journal

• Review of Scientific Instruments

  Review of Scientific Instruments 43 (3), 404-409, 1972-03-01

  AIP Publishing

Keywords

• Instrumentation

Details

• CRID: 1362262944432311936
• NII Article ID: 30015964618
• DOI: 10.1063/1.1685647
• ISSN: 10897623
• ISSN: 00346748
• Web Site: https://pubs.aip.org/aip/rsi/article-pdf/43/3/404/19177575/404_1_online.pdf
• Data Source:
  • Crossref
  • CiNii Articles