Proliferation and migration of primordial germ cells in <i>W</i>^<i>e</i>/<i>W</i>^<i>e</i> mouse embryos

• Mia Buehr

• Anne McLaren

• Aine Bartley

• Susan Darling

抄録

We have examined the numbers and distribution of primordial germ cells in W e / W e , W e /+, and +/+ mouse embryos using Southern blotting to determine embryo genotypes. At early somite stages (5–7 somites: approximately 81/2 days post coitum [dpc]) there are 50 to 100 germ cells in embryos of all genotypes. The number of germ cells in W e /+ and +/+ embryos then begins to increase: at later somite stages (17–19 somites: approximately 91/2 dpc) they number about 200, and by 101/2 dpc there are approximately 725 W e /+ and 850 +/+ germ cells. During this time, however, the number of germ cells in W e / W e embryos remains less than 100. At 81/2 dec, the distribution of germ cells in the hindgut endoderm is the same in all genotypes. By 91/2 dpc, 30% of W e / W e germ cells are found in ectopic sites (allantois and vitelline artery); germ cell distribution along the length of the hindgut appears normal, but germ cells remain confined to the floor of the gut in W e / W e embryos, rather than being distributed around its circumference as in the other two genotypes. By 101/2 days, the migration of W e / W e germ cells through the dorsal mesentery lags behind that of the other genotypes, and a larger proportion remains in the gut wall. © 1993 Wiley‐Liss, Inc.

収録刊行物

• Developmental Dynamics

  Developmental Dynamics 198 (3), 182-189, 1993-11

  Wiley

キーワード

• Developmental Biology

詳細情報

• CRID: 1361418520560365056
• NII論文ID: 30016205062
• NII書誌ID: AA1082160X
• DOI: 10.1002/aja.1001980304
• ISSN: 10970177
• ISSN: 10588388
• Web Site: https://api.wiley.com/onlinelibrary/tdm/v1/articles/10.1002%2Faja.1001980304
• Web Site: https://onlinelibrary.wiley.com/doi/full/10.1002/aja.1001980304
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