The C-proteinase that processes procollagens to fibrillar collagens is identical to the protein previously identified as bone morphogenic protein-1.

• S W Li

  Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.

• A L Sieron

  Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.

• A Fertala

  Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.

• Y Hojima

  Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.

• W V Arnold

  Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.

• D J Prockop

  Department of Biochemisty and Molcular Biology, Jefferson Medical College, Thomas Jefferson University, Philadelphia, PA 19107, USA.

抄録

Bone morphogenic protein-1 (BMP-1) was originally identified as one of several BMPs that induced new bone formation when implanted into ectopic sites in rodents. BMP-1, however, differed from other BMPs in that it its structure was not similar to transforming growth factor beta. Instead, it had a large domain homologous to a metalloendopeptidase isolated from crayfish, an epidermal growth-factor-like domain, and three regions of internal sequence homology referred to as CUB domains. Therefore, BMP-1 was a member of the "astacin families" of zinc-requiring endopeptidases. Many astacins have been shown to play critical roles in embryonic hatching, dorsal/ventral patterning, and early developmental decisions. Here, we have obtained amino acid sequences and isolated cDNA clones for procollagen C-proteinase (EC 3.4.24.19), an enzyme that is essential for the processing of procollagens to fibrillar collagens. The results demonstrate that procollagen C-proteinase is identical to BMP-1.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 93 (10), 5127-5130, 1996-05-14

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361981470609722368
• NII論文ID: 30016220730
• DOI: 10.1073/pnas.93.10.5127
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.93.10.5127
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