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- Matthew K. Higgins
- Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom; and Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
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- Evert Bokma
- Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom; and Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
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- Eva Koronakis
- Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom; and Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
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- Colin Hughes
- Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom; and Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
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- Vassilis Koronakis
- Department of Pathology, Cambridge University, Tennis Court Road, Cambridge CB2 1QP, United Kingdom; and Medical Research Council Laboratory of Molecular Biology, Hills Road, Cambridge CB2 2QH, United Kingdom
抄録
<jats:p> Multidrug resistance among Gram-negative bacteria is conferred by three-component membrane pumps that expel diverse antibiotics from the cell. These efflux pumps consist of an inner membrane transporter such as the AcrB proton antiporter, an outer membrane exit duct of the TolC family, and a periplasmic protein known as the adaptor. We present the x-ray structure of the MexA adaptor from the human pathogen <jats:italic>Pseudomonas aeruginosa</jats:italic> . The elongated molecule contains three linearly arranged subdomains; a 47-Å-long α-helical hairpin, a lipoyl domain, and a six-stranded β-barrel. In the crystal, hairpins of neighboring MexA monomers pack side-by-side to form twisted arcs. We discuss the implications of the packing of molecules within the crystal. On the basis of the structure and packing, we suggest a model for the key periplasmic interaction between the outer membrane channel and the adaptor protein in the assembled drug efflux pump. </jats:p>
収録刊行物
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- Proceedings of the National Academy of Sciences
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Proceedings of the National Academy of Sciences 101 (27), 9994-9999, 2004-06-28
Proceedings of the National Academy of Sciences
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キーワード
詳細情報 詳細情報について
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- CRID
- 1360574094013129344
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- NII論文ID
- 30016241196
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- ISSN
- 10916490
- 00278424
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- データソース種別
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