Long-range compaction and flexibility of interphase chromatin in budding yeast analyzed by high-resolution imaging techniques

• Kerstin Bystricky

  Department of Molecular Biology and National Center of Competence in Research Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland; and Division of Biophysics of Macromolecules, German Cancer Research Center (Deutsche Krebsforschungszentrum), B040, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany

• Patrick Heun

  Department of Molecular Biology and National Center of Competence in Research Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland; and Division of Biophysics of Macromolecules, German Cancer Research Center (Deutsche Krebsforschungszentrum), B040, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany

• Lutz Gehlen

  Department of Molecular Biology and National Center of Competence in Research Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland; and Division of Biophysics of Macromolecules, German Cancer Research Center (Deutsche Krebsforschungszentrum), B040, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany

• Jörg Langowski

  Department of Molecular Biology and National Center of Competence in Research Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland; and Division of Biophysics of Macromolecules, German Cancer Research Center (Deutsche Krebsforschungszentrum), B040, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany

• Susan M. Gasser

  Department of Molecular Biology and National Center of Competence in Research Frontiers in Genetics, University of Geneva, 30 Quai Ernest Ansermet, 1211 Geneva, Switzerland; and Division of Biophysics of Macromolecules, German Cancer Research Center (Deutsche Krebsforschungszentrum), B040, Im Neuenheimer Feld 580, 69120 Heidelberg, Germany

抄録

Little is known about how chromatin folds in its native state. Using optimized in situ hybridization and live imaging techniques have determined compaction ratios and fiber flexibility for interphase chromatin in budding yeast. Unlike previous studies, ours examines nonrepetitive chromatin at intervals short enough to be meaningful for yeast chromosomes and functional domains in higher eukaryotes. We reconcile high-resolution fluorescence in situ hybridization data from intervals of 14–100 kb along single chromatids with measurements of whole chromosome arms (122–623 kb in length), monitored in intact cells through the targeted binding of bacterial repressors fused to GFP derivatives. The results are interpreted with a flexible polymer model and suggest that interphase chromatin exists in a compact higher-order conformation with a persistence length of 170–220 nm and a mass density of ≈110–150 bp/nm. These values are equivalent to 7–10 nucleosomes per 11-nm turn within a 30-nm-like fiber structure. Comparison of long and short chromatid arm measurements demonstrates that chromatin fiber extension is also influenced by nuclear geometry. The observation of this surprisingly compact chromatin structure for transcriptionally competent chromatin in living yeast cells suggests that the passage of RNA polymerase II requires a very transient unfolding of higher-order chromatin structure.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 101 (47), 16495-16500, 2004-11-15

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1362544420263690240
• NII論文ID: 30016244425
• DOI: 10.1073/pnas.0402766101
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0402766101
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