Genomic imprinting of XX spermatogonia and XX oocytes recovered from XX↔XY chimeric testes

• Ayako Isotani

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Tomoko Nakanishi

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Shin Kobayashi

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Jiyoung Lee

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Shinichiro Chuma

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Norio Nakatsuji

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Fumitoshi Ishino

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

• Masaru Okabe

  Genome Information Research Center and Graduate School of Pharmaceutical Sciences, Osaka University, 3-1 Yamadaoka, Suita, Osaka 565-0871, Japan; Gene Research Center, Tokyo Institute of Technology, Kanagawa 226-8501, Japan; Core Research for Evolutional Science and Technology, Japan Science and Technology Corporation, Chuou-ku, Tokyo 113-0027, Japan; and Department of Development and Differentiation, Institute for Frontier Medical Sciences, Kyoto University, 53 Kawahara-cho Shogoin, Sakyo-ku, Kyoto...

抄録

We produced XX↔XY chimeras by using embryos whose X chromosomes were tagged with EGFP (X*), making the fluorescent green female (XX*) germ cells easily distinguishable from their nonfluorescent male (XY) counterparts. Taking advantage of tagging with EGFP, the XX* “prospermatogonia” were isolated from the testes, and the status of their genomic imprinting was examined. It was shown that these XX cells underwent a paternal imprinting, despite their chromosomal constitution. As previously indicated in sex-reversal XXsxr testes, we also found a few green XX* germ cells developed as “eggs” within the seminiferous tubules of XX*↔XY chimeric testes. These cells were indistinguishable from XX* prospermatogonia at birth but resumed oogenesis in a testicular environment. The biological nature of the “testicular eggs” was examined by recovering the eggs from chimeric testes. The testicular eggs not only formed an egg-specific structure, the zona pellucida, but also were able to fuse with sperm. The collected testicular eggs were indicated to undergo maternal imprinting, despite the testicular environment. The genomic imprinting did not always follow the environmental conditions of where the germ cells resided; rather, it was defined by the sex that was chosen by the germ cells at early embryonic stage.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 102 (11), 4039-4044, 2005-03-03

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855569337692544
• NII論文ID: 30016245486
• DOI: 10.1073/pnas.0406769102
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.0406769102
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