FORMATION AND DETECTION OF RNA-DNA HYBRID MOLECULES IN CYTOLOGICAL PREPARATIONS

• Joseph G. Gall

  KLINE BIOLOGY TOWER, YALE UNIVERSITY

• Mary Lou Pardue

  KLINE BIOLOGY TOWER, YALE UNIVERSITY

抄録

A technique is described for forming molecular hybrids between RNA in solution and the DNA of intact cytological preparations. Cells in a conventional tissue squash are immobilized under a thin layer of agar. Next they are treated with alkali to denature the DNA and then incubated with tritium-labeled RNA. The hybrids are detected by autoradiography. The technique is illustrated by the hybridization of ribosomal RNA to the amplified ribosomal genes in oocytes of the toad Xenopus . A low level of gene amplification was also detected in premeiotic nuclei (oogonia).

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 63 (2), 378-383, 1969-06

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361981470561098240
• NII論文ID: 30016257252
• DOI: 10.1073/pnas.63.2.378
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.63.2.378
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