A Thrombin-Sensitive Protein of Human Platelet Membranes

• Nancy Lewis Baenziger

  Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

• G. N. Brodie

  Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

• Philip W. Majerus

  Department of Internal Medicine, Washington University School of Medicine, St. Louis, Missouri 63110

抄録

The action of thrombin on intact human platelets has been studied with the aid of polyacrylamide gel electrophoresis in sodium dodecylsulfate. A single major membrane protein band with a molecular weight of 190,000 disappears after thrombin treatment, while a new membrane protein with a molecular weight of 107,000 appears. This may represent hydrolysis of the thrombin-sensitive protein. When platelets are disrupted or when the thrombin-sensitive protein is solubilized from membranes prior to thrombin treatment, no hydrolysis occurs. The effect of thrombin on the platelet membrane protein is complete within 2 min which suggests that hydrolysis of this membrane protein may trigger the physiological effects of thrombin on platelets.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 68 (1), 240-243, 1971-01

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1362544421263432448
• NII論文ID: 30016258284
• DOI: 10.1073/pnas.68.1.240
• ISSN: 10916490
• ISSN: 00278424
• ISSN: http://id.crossref.org/issn/00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.68.1.240
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