Detection of DNA Synthesis in Intact Organisms with Positron-Emitting [ <i>Methyl</i> - ¹¹ C]Thymidine

• David Christman

  Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973

• Elizabeth J. Crawford

  Department of Biology, University of California, San Diego, La Jolla, Calif. 92037

• Morris Friedkin

  Department of Biology, University of California, San Diego, La Jolla, Calif. 92037

• Alfred P. Wolf

  Chemistry Department, Brookhaven National Laboratory, Upton, New York 11973

抄録

11 CO 2 produced in the Brookhaven 152-cm cyclotron was converted to formaldehyde, which in turn was used for the enzymatic conversion of deoxyuridine-5′-phosphate to [ 11 C]thymidylate. Enzymatic treatment of the nucleotide with alkaline phosphatase gave [ 11 C]thymidine. The preparation of [ 11 C]thymidine from cyclotron-generated 11 CO 2 required 110 min (about 5 half-lives): 35 min for the synthesis of H 11 CHO, 25 min for the enzymatic conversion to [ 11 C]thymidylate, 20 min for column chromatography, 5 min for phosphatase treatment, 10 min for evaporation, 2 min for filtration through an anion-exchange resin, and 13 min for miscellaneous manipulations. Positron-emitting [ 11 C]thymidine and [ 11 C]thymidylate were used for in vivo tracer studies of DNA synthesis in mice for periods of up to 3 hr. Findings with carbon-11 were consistent with earlier studies in which carbon-14 and tritium-labeled thymidine were used. For example, 3 hr after injection of [ 11 C]thymidine, spleen DNA was labeled to a much greater extent than was liver DNA.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 69 (4), 988-992, 1972-04

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361981469338569216
• NII論文ID: 30016259518
• DOI: 10.1073/pnas.69.4.988
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.69.4.988
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