K-sam, an amplified gene in stomach cancer, is a member of the heparin-binding growth factor receptor genes.

• Y Hattori

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• H Odagiri

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• H Nakatani

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• K Miyagawa

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• K Naito

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• H Sakamoto

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• O Katoh

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• T Yoshida

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• T Sugimura

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

• M Terada

  Genetics Division, National Cancer Center Research Institute, Tokyo, Japan.

抄録

DNA fragments amplified in a stomach cancer-derived cell line, KATO-III, were previously identified by the in-gel DNA renaturation method, and a 0.2-kilobase-pair fragment of the amplified sequence was subsequently cloned. By genomic walking, a portion of the exon of the gene flanking this 0.2-kilobase-pair fragment was cloned, and the gene was designated as K-sam (KATO-III cell-derived stomach cancer amplified gene). The K-sam cDNAs, corresponding to the 3.5-kilobase K-sam mRNA, were cloned from the KATO-III cells. Sequence analysis revealed that this gene coded for 682 amino acid residues that satisfied the characteristics of the receptor tyrosine kinase. The K-sam gene had significant homologies with bek, FLG, and chicken basic fibroblast growth factor receptor gene. The K-sam gene was amplified in KATO-III cells with the major transcript of 3.5-kilobases in size. This gene was also expressed in some other stomach cancer cells, a small cell lung cancer, and germ cell tumors.

収録刊行物

• Proceedings of the National Academy of Sciences

  Proceedings of the National Academy of Sciences 87 (15), 5983-5987, 1990-08

  Proceedings of the National Academy of Sciences

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360855569327158784
• NII論文ID: 30016287579
• DOI: 10.1073/pnas.87.15.5983
• ISSN: 10916490
• ISSN: 00278424
• Web Site: https://pnas.org/doi/pdf/10.1073/pnas.87.15.5983
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