特集：　ＳＬ‐Ｊ／ＦＭＰＴライフサイエンス実験の体験記録　無重力を利用した酵素の結晶成長

• 相原 茂夫

  京都大学食糧科学研究所

• 森田 雄平

  不二製油株式会社

書誌事項

タイトル別名

• Lessons from Sl-j/fmpt life science experiment. Crystal Growth of Enzymes in Space Microgravity.

抄録

This experiment was conducted using the microgravity environment in space to prepare single crystals of good crystallinity in which protein molecules are very well ordered. Five kinds of protein samples: hen egg-white lysozyme (2 different crystallization conditions), horse skeletal myoglobin, ω-Amino acid: pyruvate aminotransferase from Pseudomonas sp. F-126, and Rhizopus niveus lipase, were used for crystallization, which was carried out according to the batch method for 7days at 20°C. Among these protein samples, crystals were produced from the four samples and the particularly noteworthy results could be obtained from lysozyme at pH4.5 and ω-amino acid:pyruvate aminotransferase. In this space experiment, crystal growth proceeded at a slower rate than expected, compared with a control experiment on earth. Furthermore, it was proved that space-grown single crystals possessed a good crystallinity although they were not always of an optimal shape and size for X-ray crystallography. Depending on the protein samples and the crystallization conditions, crystal nuclei grew in different shapes of crystals from those of ground-grown ones due to the influence of the gravity field in space.

収録刊行物

• Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ　ｉｎ　Ｓｐａｃｅ

  Ｂｉｏｌｏｇｉｃａｌ　Ｓｃｉｅｎｃｅｓ　ｉｎ　Ｓｐａｃｅ 7 (4), 341-352, 1993

  日本宇宙生物科学会

キーワード

• General Medicine

詳細情報

• CRID: 1390282679407574912
• NII論文ID: 130004845179
• NII論文ID: 30016942318
• NII書誌ID: AN10164806
• DOI: 10.2187/bss.7.341
• ISSN: 1349967X
• ISSN: 09149201
• 本文言語コード: ja
• データソース種別:
  • JaLC
  • Crossref
  • CiNii Articles
• 抄録ライセンスフラグ: 使用不可