Rab6 Coordinates a Novel Golgi to ER Retrograde Transport Pathway in Live Cells

DOI PDF 10 Citations
  • Jamie White
    aLight Microscopy Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
  • Ludger Johannes
    cLaboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, CNRS UMR 144, Paris, Cedex 05, France
  • Frédéric Mallard
    cLaboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, CNRS UMR 144, Paris, Cedex 05, France
  • Andreas Girod
    bCell Biophysics and Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
  • Stephan Grill
    aLight Microscopy Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
  • Sigrid Reinsch
    bCell Biophysics and Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
  • Patrick Keller
    bCell Biophysics and Cell Biology Programme, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany
  • Barbara Tzschaschel
    dGBF-National Research Institute for Biotechnology, Department of Microbiology, Mascheroder Weg 1, 38124 Braunschweig, Germany
  • Arnaud Echard
    cLaboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, CNRS UMR 144, Paris, Cedex 05, France
  • Bruno Goud
    cLaboratoire Mécanismes moléculaires du transport intracellulaire, Institut Curie, CNRS UMR 144, Paris, Cedex 05, France
  • Ernst H.K. Stelzer
    aLight Microscopy Group, European Molecular Biology Laboratory, Meyerhofstrasse 1, D-69117 Heidelberg, Germany

Abstract

<jats:p>We visualized a fluorescent-protein (FP) fusion to Rab6, a Golgi-associated GTPase, in conjunction with fluorescent secretory pathway markers. FP-Rab6 defined highly dynamic transport carriers (TCs) translocating from the Golgi to the cell periphery. FP-Rab6 TCs specifically accumulated a retrograde cargo, the wild-type Shiga toxin B-fragment (STB), during STB transport from the Golgi to the endoplasmic reticulum (ER). FP-Rab6 TCs associated intimately with the ER, and STB entered the ER via specialized peripheral regions that accumulated FP-Rab6. Microinjection of antibodies that block coatomer protein I (COPI) function inhibited trafficking of a KDEL-receptor FP-fusion, but not FP-Rab6. Additionally, markers of COPI-dependent recycling were excluded from FP-Rab6/STB TCs. Overexpression of Rab6:GDP (T27N mutant) using T7 vaccinia inhibited toxicity of Shiga holotoxin, but did not alter STB transport to the Golgi or Golgi morphology. Taken together, our results indicate Rab6 regulates a novel Golgi to ER transport pathway.</jats:p>

Journal

Citations (10)*help

See more

Keywords

Details 詳細情報について

Report a problem

Back to top