FIXATION OF NEURAL TISSUES FOR ELECTRON MICROSCOPY BY PERFUSION WITH SOLUTIONS OF OSMIUM TETROXIDE

DOI PDF 被引用文献4件
  • Sanford L. Palay
    From the Laboratory of Neuroanatomical Sciences, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland.
  • S. M. McGee-Russell
    From the Laboratory of Neuroanatomical Sciences, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland.
  • Spencer Gordon
    From the Laboratory of Neuroanatomical Sciences, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland.
  • Mary A. Grillo
    From the Laboratory of Neuroanatomical Sciences, National Institute of Neurological Diseases and Blindness, National Institutes of Health, Bethesda, Maryland.

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<jats:p>This paper describes in detail a method for obtaining nearly uniform fixation of the nervous system by vascular perfusion with solutions of osmium tetroxide. Criteria are given for evaluating the degree of success achieved in the preservation of all the cellular components of the nervous system. The method permits analysis of the structural relations between cells at the electron microscopic level to an extent that has not been possible heretofore.</jats:p>

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