Commitment and Differentiation of Osteoclast Precursor Cells by the Sequential Expression of C-Fms and Receptor Activator of Nuclear Factor κb (Rank) Receptors
-
- Fumio Arai
- aDepartment of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan
-
- Takeshi Miyamoto
- aDepartment of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan
-
- Osamu Ohneda
- aDepartment of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan
-
- Tomohisa Inada
- aDepartment of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan
-
- Tetsuo Sudo
- cBasic Research Laboratories, Toray Industries, Incorporated, Kamakura 248-0036, Japan
-
- Kenneth Brasel
- dDepartment of Molecular Biology, Immunex Corporation, Seattle, Washington 98101-2936
-
- Takashi Miyata
- bDepartment of Periodontology, Meikai University School of Dentistry, Sakado 350-0248, Japan
-
- Dirk M. Anderson
- dDepartment of Molecular Biology, Immunex Corporation, Seattle, Washington 98101-2936
-
- Toshio Suda
- aDepartment of Cell Differentiation, Institute of Molecular Embryology and Genetics, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan
抄録
<jats:p>Osteoclasts are terminally differentiated cells derived from hematopoietic stem cells. However, how their precursor cells diverge from macrophagic lineages is not known. We have identified early and late stages of osteoclastogenesis, in which precursor cells sequentially express c-Fms followed by receptor activator of nuclear factor κB (RANK), and have demonstrated that RANK expression in early-stage of precursor cells (c-Fms+RANK−) was stimulated by macrophage colony-stimulating factor (M-CSF). Although M-CSF and RANKL (ligand) induced commitment of late-stage precursor cells (c-Fms+RANK+) into osteoclasts, even late-stage precursors have the potential to differentiate into macrophages without RANKL. Pretreatment of precursors with M-CSF and delayed addition of RANKL showed that timing of RANK expression and subsequent binding of RANKL are critical for osteoclastogenesis. Thus, the RANK–RANKL system determines the osteoclast differentiation of bipotential precursors in the default pathway of macrophagic differentiation.</jats:p>
収録刊行物
-
- The Journal of Experimental Medicine
-
The Journal of Experimental Medicine 190 (12), 1741-1754, 1999-12-20
Rockefeller University Press
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1362544418716541184
-
- NII論文ID
- 30017414690
-
- ISSN
- 15409538
- 00221007
-
- データソース種別
-
- Crossref
- CiNii Articles