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- Alfonso Martín-Fontecha
- 1Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland
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- Silvia Sebastiani
- 1Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland
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- Uta E. Höpken
- 2Max Delbrück Center for Molecular Medicine, 13122 Berlin-Buch, Germany
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- Mariagrazia Uguccioni
- 1Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland
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- Martin Lipp
- 2Max Delbrück Center for Molecular Medicine, 13122 Berlin-Buch, Germany
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- Antonio Lanzavecchia
- 1Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland
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- Federica Sallusto
- 1Institute for Research in Biomedicine, CH-6500 Bellinzona, Switzerland
抄録
<jats:p>Antigen-pulsed dendritic cells (DCs) are used as natural adjuvants for vaccination, but the factors that influence the efficacy of this treatment are poorly understood. We investigated the parameters that affect the migration of subcutaneously injected mouse-mature DCs to the draining lymph node. We found that the efficiency of DC migration varied with the number of injected DCs and that CCR7+/+ DCs migrating to the draining lymph node, but not CCR7−/− DCs that failed to do so, efficiently induced a rapid increase in lymph node cellularity, which was observed before the onset of T cell proliferation. We also report that DC migration could be increased up to 10-fold by preinjection of inflammatory cytokines that increased the expression of the CCR7 ligand CCL21 in lymphatic endothelial cells. The magnitude and quality of CD4+ T cell response was proportional to the number of antigen-carrying DCs that reached the lymph node and could be boosted up to 40-fold by preinjection of tumor necrosis factor that conditioned the tissue for increased DC migration. These results indicate that DC number and tissue inflammation are critical parameters for DC-based vaccination.</jats:p>
収録刊行物
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- The Journal of Experimental Medicine
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The Journal of Experimental Medicine 198 (4), 615-621, 2003-08-18
Rockefeller University Press
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詳細情報 詳細情報について
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- CRID
- 1363388843800258176
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- NII論文ID
- 30017417208
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- ISSN
- 15409538
- 00221007
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- データソース種別
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