CHARACTERISTICS OF GROWTH OF SARCOMA AND CARCINOMA CULTIVATED IN VITRO

  • Robert A. Lambert
    From the Department of Pathology of the College of Physicians and Surgeons, Columbia University, New York.
  • Frederic M. Hanes
    From the Department of Pathology of the College of Physicians and Surgeons, Columbia University, New York.

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<jats:p>1. The transplantable sarcomata of rats and mice grow very readily by the method of cultivating tissues in vitro.</jats:p> <jats:p>2. Sarcomatous tissue grows in conformity to a type which may be regarded as characteristic for tissues of mesenchymal origin.</jats:p> <jats:p>3. The growth of sarcoma cells in vitro consists in ameboid wandering into the surrounding plasma, karyokinetic proliferation. and evidences of active metabolism on the part of the cells.</jats:p> <jats:p>4. Mouse carcinomata can be cultivated in vitro. The outgrowth of carcinoma cells assumes a sheet-like form, only one cell in thickness. They migrate into the plasma by ameboid movement, the advancing edge showing numerous prolongations of the cytoplasm into pseudopods.</jats:p> <jats:p>5. Karyokinetic figures are frequently seen in growing carcinoma cells. The cells show evidences of active metabolism.</jats:p> <jats:p>6. Both sarcoma and carcinoma cells cultivated in vitro show active phagocytosis; carmin particles placed in the plasma are taken up rapidly by the growing cells.</jats:p>

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