Distinct LIN-10 Domains Are Required for Its Neuronal Function, Its Epithelial Function, and Its Synaptic Localization

• Doreen R. Glodowski

  The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

• Tricia Wright

  The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

• Keri Martinowich

  The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

• Howard Chia-Hao Chang

  The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

• Douglas Beach

  The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

• Christopher Rongo

  The Waksman Institute, Department of Genetics, Rutgers University, Piscataway, NJ 08854

抄録

α-Amino-3-hydroxy-5-methyl-4-isoxazole propionic acid (AMPA)-type glutamate receptors (AMPARs) mediate excitatory neurotransmission at neuronal synapses, and their regulated localization plays a role in synaptic plasticity. In Caenorhabditis elegans, the PDZ and PTB domain-containing protein LIN-10 is required both for the synaptic localization of the AMPAR subunit GLR-1 and for vulval fate induction in epithelia. Here, we examine the role that different LIN-10 domains play in GLR-1 localization. We find that an amino-terminal region of LIN-10 directs LIN-10 protein localization to the Golgi and to synaptic clusters. In addition, mutations in the carboxyl-terminal PDZ domains prevent LIN-10 from regulating GLR-1 localization in neurons but do not prevent LIN-10 from functioning in the vulval epithelia. A mutation in the amino terminus prevents the protein from functioning in the vulval epithelia but does not prevent it from functioning to regulate GLR-1 localization in neurons. Finally, we show that human Mint2 can substitute for LIN-10 to facilitate GLR-1 localization in neurons and that the Mint2 amino terminus is critical for this function. Together, our data suggest that LIN-10 uses distinct modular domains for its functions in neurons and epithelial cells and that during evolution its vertebrate ortholog Mint2 has retained the ability to direct AMPAR localization in neurons.

収録刊行物

• Molecular Biology of the Cell

  Molecular Biology of the Cell 16 (3), 1417-1426, 2005-03

  American Society for Cell Biology (ASCB)

キーワード

• Cell Biology
• Molecular Biology

詳細情報

• CRID: 1361418521463330816
• NII論文ID: 30018379773
• DOI: 10.1091/mbc.e04-10-0885
• ISSN: 19394586
• ISSN: 10591524
• データソース種別:
  • Crossref
  • CiNii Articles