A Minus-End–directed Kinesin with Plus-End Tracking Protein Activity Is Involved in Spindle Morphogenesis
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- J. Christian Ambrose
- The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802
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- Wuxing Li
- Plant Physiology Program, The Pennsylvania State University, University Park, PA 16802
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- Adam Marcus
- Department of Biology, The Pennsylvania State University, University Park, PA 16802
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- Hong Ma
- Department of Biology, The Pennsylvania State University, University Park, PA 16802
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- Richard Cyr
- Department of Biology, The Pennsylvania State University, University Park, PA 16802
抄録
<jats:p>Diverse kinesin motor proteins are involved in spindle function; however, the mechanisms by which they are targeted to specific sites within spindles are not well understood. Here, we show that a fusion between yellow fluorescent protein (YFP) and a minus-end–directed Kinesin-14 (C-terminal family) from Arabidopsis, ATK5, localizes to mitotic spindle midzones and regions rich in growing plus-ends within phragmoplasts. Notably, in Arabidopsis interphase cells, YFP::ATK5 localizes to microtubules with a preferential enrichment at growing plus-ends; indicating ATK5 is a plus-end tracking protein (+TIP). This +TIP activity is conferred by regions outside of the C-terminal motor domain, which reveals the presence of independent plus-end tracking and minus-end motor activities within ATK5. Furthermore, mitotic spindles of atk5 null mutant plants are abnormally broadened. Based on these data, we propose a model in which ATK5 uses plus-end tracking to reach spindle midzones, where it then organizes microtubules via minus-end–directed motor activity.</jats:p>
収録刊行物
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- Molecular Biology of the Cell
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Molecular Biology of the Cell 16 (4), 1584-1592, 2005-04
American Society for Cell Biology (ASCB)
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詳細情報
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- CRID
- 1364233271011225216
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- NII論文ID
- 30018379787
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- ISSN
- 19394586
- 10591524
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