A Minus-End–directed Kinesin with Plus-End Tracking Protein Activity Is Involved in Spindle Morphogenesis

  • J. Christian Ambrose
    The Huck Institutes of the Life Sciences, The Pennsylvania State University, University Park, PA 16802
  • Wuxing Li
    Plant Physiology Program, The Pennsylvania State University, University Park, PA 16802
  • Adam Marcus
    Department of Biology, The Pennsylvania State University, University Park, PA 16802
  • Hong Ma
    Department of Biology, The Pennsylvania State University, University Park, PA 16802
  • Richard Cyr
    Department of Biology, The Pennsylvania State University, University Park, PA 16802

抄録

<jats:p>Diverse kinesin motor proteins are involved in spindle function; however, the mechanisms by which they are targeted to specific sites within spindles are not well understood. Here, we show that a fusion between yellow fluorescent protein (YFP) and a minus-end–directed Kinesin-14 (C-terminal family) from Arabidopsis, ATK5, localizes to mitotic spindle midzones and regions rich in growing plus-ends within phragmoplasts. Notably, in Arabidopsis interphase cells, YFP::ATK5 localizes to microtubules with a preferential enrichment at growing plus-ends; indicating ATK5 is a plus-end tracking protein (+TIP). This +TIP activity is conferred by regions outside of the C-terminal motor domain, which reveals the presence of independent plus-end tracking and minus-end motor activities within ATK5. Furthermore, mitotic spindles of atk5 null mutant plants are abnormally broadened. Based on these data, we propose a model in which ATK5 uses plus-end tracking to reach spindle midzones, where it then organizes microtubules via minus-end–directed motor activity.</jats:p>

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詳細情報

  • CRID
    1364233271011225216
  • NII論文ID
    30018379787
  • DOI
    10.1091/mbc.e04-10-0935
  • ISSN
    19394586
    10591524
  • データソース種別
    • Crossref
    • CiNii Articles

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