The Arabidopsis<i>SKU6</i>/<i>SPIRAL1</i>Gene Encodes a Plus End–Localized Microtubule-Interacting Protein Involved in Directional Cell Expansion[W]
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- John C. Sedbrook
- Department of Biological Sciences, Illinois State University, Normal, Illinois 61790
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- David W. Ehrhardt
- Carnegie Institution, Stanford, California 94305
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- Sarah E. Fisher
- Carnegie Institution, Stanford, California 94305
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- Wolf-Rüdiger Scheible
- Max Planck Institute of Molecular Plant Physiology, 14476 Golm, Germany
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- Chris R. Somerville
- Carnegie Institution, Stanford, California 94305
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<jats:title>Abstract</jats:title><jats:p>The sku6-1 mutant of Arabidopsis thaliana exhibits altered patterns of root and organ growth. sku6 roots, etiolated hypocotyls, and leaf petioles exhibit right-handed axial twisting, and root growth on inclined agar media is strongly right skewed. The touch-dependent sku6 root skewing phenotype is suppressed by the antimicrotubule drugs propyzamide and oryzalin, and right skewing is exacerbated by cold treatment. Cloning revealed that sku6-1 is allelic to spiral1-1 (spr1-1). However, modifiers in the Columbia (Col) and Landsberg erecta (Ler) ecotype backgrounds mask noncomplementation in sku6-1 (Col)/spr1-1 (Ler) F1 plants. The SPR1 gene encodes a plant-specific 12-kD protein that is ubiquitously expressed and belongs to a six-member gene family in Arabidopsis. An SPR1:green fluorescent protein (GFP) fusion expressed in transgenic seedlings localized to microtubules within the cortical array, preprophase band, phragmoplast, and mitotic spindle. SPR1:GFP was concentrated at the growing ends of cortical microtubules and was dependent on polymer growth state; the microtubule-related fluorescence dissipated upon polymer shortening. The protein has a repeated motif at both ends, separated by a predicted rod-like domain, suggesting that it may act as an intermolecular linker. These observations suggest that SPR1 is involved in microtubule polymerization dynamics and/or guidance, which in turn influences touch-induced directional cell expansion and axial twisting.</jats:p>
収録刊行物
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- The Plant Cell
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The Plant Cell 16 (6), 1506-1520, 2004-06-01
Oxford University Press (OUP)
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詳細情報 詳細情報について
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- CRID
- 1364233271274582912
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- NII論文ID
- 30019357119
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- ISSN
- 1532298X
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- データソース種別
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