Encounters between Dynamic Cortical Microtubules Promote Ordering of the Cortical Array through Angle-Dependent Modifications of Microtubule Behavior[W]

• Ram Dixit

  Penn State University, University Park, Pennsylvania 16802

• Richard Cyr

  Penn State University, University Park, Pennsylvania 16802

抄録

Ordered cortical microtubule arrays are essential for normal plant morphogenesis, but how these arrays form is unclear. The dynamics of individual cortical microtubules are stochastic and cannot fully account for the observed order; however, using tobacco (Nicotiana tabacum) cells expressing either the MBD-DsRed (microtubule binding domain of the mammalian MAP4 fused to the Discosoma sp red fluorescent protein) or YFP-TUA6 (yellow fluorescent protein fused to the Arabidopsis α-tubulin 6 isoform) microtubule markers, we identified intermicrotubule interactions that modify their stochastic behaviors. The intermicrotubule interactions occur when the growing plus-ends of cortical microtubules encounter previously existing cortical microtubules. Importantly, the outcome of such encounters depends on the angle at which they occur: steep-angle collisions are characterized by approximately sevenfold shorter microtubule contact times compared with shallow-angle encounters, and steep-angle collisions are twice as likely to result in microtubule depolymerization. Hence, steep-angle collisions promote microtubule destabilization, whereas shallow-angle encounters promote both microtubule stabilization and coalignment. Monte Carlo modeling of the behavior of simulated microtubules, according to the observed behavior of transverse and longitudinally oriented cortical microtubules in cells, reveals that these simple rules for intermicrotubule interactions are necessary and sufficient to facilitate the self-organization of dynamic microtubules into a parallel configuration.

収録刊行物

• The Plant Cell

  The Plant Cell 16 (12), 3274-3284, 2004-12-01

  Oxford University Press (OUP)

キーワード

• Cell Biology
• Plant Science

詳細情報

• CRID: 1361137045486493696
• NII論文ID: 30019357423
• DOI: 10.1105/tpc.104.026930
• ISSN: 1532298X
• Web Site: http://academic.oup.com/plcell/article-pdf/16/12/3274/36875841/plcell_v16_12_3274.pdf
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