Accumulation of a Clock-Regulated Transcript during Flower-Inductive Darkness in <i>Pharbitis nil</i>1

DOI PDF 被引用文献12件
  • Kimiyo Sage-Ono
    Gene Experiment Center, University of Tsukuba, Tsukuba, Ibaraki, 305–8572 Japan (K.S.O., H.H., H.K.)
  • Michiyuki Ono
    Biotechnology Institute, Akita Prefectural College of Agriculture, Ohgata, Akita, 010–0444 Japan (M.O.)
  • Hiroshi Harada
    Gene Experiment Center, University of Tsukuba, Tsukuba, Ibaraki, 305–8572 Japan (K.S.O., H.H., H.K.)
  • Hiroshi Kamada
    Gene Experiment Center, University of Tsukuba, Tsukuba, Ibaraki, 305–8572 Japan (K.S.O., H.H., H.K.)

抄録

<jats:title>Abstract</jats:title> <jats:p>To clarify the molecular basis of the photoperiodic induction of flowering in the short-day plantPharbitis nil cv Violet, we examined changes in the level of mRNA in cotyledons during the flower-inductive photoperiod using the technique of differential display by the polymerase chain reaction. A transcript that accumulated during the inductive dark period was identified and a cDNA corresponding to the transcript, designated PnC401 (P. nil C401), was isolated. RNA-blot hybridization verified that levels ofPnC401 mRNA fluctuated with a circadian rhythm, with maxima between 12 and 16 h after the beginning of the dark period) and minima of approximately 0. This oscillation continued even during an extended dark period but was damped under continuous light. Accumulation of PnC401 mRNA was reduced by a brief exposure to red light at the 8th h of the dark period (night-break treatment) or by exposure to far-red light at the end of the light period (end-of-day far-red treatment). These results suggest that fluctuations in levels of PnC401 mRNA are regulated by phytochrome(s) and a circadian clock and that they are associated with photoperiodic events that include induction of flowering.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 116 (4), 1479-1485, 1998-04-01

    Oxford University Press (OUP)

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