Regulation of Gibberellin 20-Oxidase and Gibberellin 3β-Hydroxylase Transcript Accumulation during De-Etiolation of Pea Seedlings

DOI PDF 被引用文献6件
  • Tahar Ait-Ali
    Laboratory for Plant Hormone Function (T.A.-A., Y.K.) and
  • Shannon Frances
    Laboratory for Photoperception and Signal Transduction (S.F., R.E.K.), Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351–0198, Japan; and
  • James L. Weller
    Department of Plant Science, University of Tasmania, G.P.O. Box 252–55, Hobart, Tasmania 7001, Australia (J.L.W., J.B.R.)
  • James B. Reid
    Department of Plant Science, University of Tasmania, G.P.O. Box 252–55, Hobart, Tasmania 7001, Australia (J.L.W., J.B.R.)
  • Richard E. Kendrick
    Laboratory for Photoperception and Signal Transduction (S.F., R.E.K.), Frontier Research Program, The Institute of Physical and Chemical Research (RIKEN), Wako-shi, Saitama 351–0198, Japan; and
  • Yuji Kamiya
    Laboratory for Plant Hormone Function (T.A.-A., Y.K.) and

抄録

<jats:title>Abstract</jats:title> <jats:p>Gibberellin (GA) 20-oxidase (GA 20-ox) and GA 3β-hydroxylase (GA 3β-hy) are enzymes that catalyze the late steps in the formation of active GAs, and are potential control points in the regulation of GA biosynthesis by light. We have investigated the photoregulation of the GA 20-ox and GA 3β-hy transcript levels in pea (Pisum sativum L.). The GA 20-oxtranscript level was higher in light-grown seedlings than in etiolated seedlings, whereas GA 3β-hy mRNA accumulation was higher in etiolated seedlings. However, transfer of etiolated seedlings to light led to a 5-fold increase in the expression of both transcripts 4 h after transfer. GA 20-ox mRNA accumulation is regulated by both phytochromes A and B. Transfer to light also resulted in a 6-fold decrease in GA1 levels within 2 h. These results suggest that the light-induced drop in GA1 level is not achieved through regulation of GA 20-ox andGA 3β-hy mRNA accumulation. The application of exogenous GA1 to apical buds of etiolated seedlings prior to light treatments inhibited the light-induced accumulation of bothGA 20-ox and GA 3β-hy mRNA, suggesting that negative feedback regulation is an important mechanism in the regulation of GA 20-ox and GA 3β-hymRNA accumulation during de-etiolation of pea seedlings.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 121 (3), 783-791, 1999-11-01

    Oxford University Press (OUP)

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