PsbP Protein, But Not PsbQ Protein, Is Essential for the Regulation and Stabilization of Photosystem II in Higher Plants

DOI PDF 被引用文献21件
  • Kentaro Ifuku
    Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan (K.I., Y.Y., S.I., F.S.); and Laboratory for Photo-Biology (1), RIKEN Photodynamics Research Center, Aoba-ku, Sendai 980–0845, Japan (T.O.)
  • Yumiko Yamamoto
    Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan (K.I., Y.Y., S.I., F.S.); and Laboratory for Photo-Biology (1), RIKEN Photodynamics Research Center, Aoba-ku, Sendai 980–0845, Japan (T.O.)
  • Taka-aki Ono
    Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan (K.I., Y.Y., S.I., F.S.); and Laboratory for Photo-Biology (1), RIKEN Photodynamics Research Center, Aoba-ku, Sendai 980–0845, Japan (T.O.)
  • Seiko Ishihara
    Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan (K.I., Y.Y., S.I., F.S.); and Laboratory for Photo-Biology (1), RIKEN Photodynamics Research Center, Aoba-ku, Sendai 980–0845, Japan (T.O.)
  • Fumihiko Sato
    Graduate School of Biostudies, Kyoto University, Sakyo-ku, Kyoto 606–8502, Japan (K.I., Y.Y., S.I., F.S.); and Laboratory for Photo-Biology (1), RIKEN Photodynamics Research Center, Aoba-ku, Sendai 980–0845, Japan (T.O.)

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<jats:title>Abstract</jats:title> <jats:p>PsbP and PsbQ proteins are extrinsic subunits of photosystem II (PSII) and participate in the normal function of photosynthetic water oxidation. Both proteins exist in a broad range of the oxygenic photosynthetic organisms; however, their physiological roles in vivo have not been well defined in higher plants. In this study, we established and analyzed transgenic tobacco (Nicotiana tabacum) plants in which the levels of PsbP or PsbQ were severely down-regulated by the RNA interference technique. A plant that lacked PsbQ showed no specific phenotype compared to a wild-type plant. This suggests that PsbQ in higher plants is dispensable under the normal growth condition. On the other hand, a plant that lacked PsbP showed prominent phenotypes: drastic retardation of growth, pale-green-colored leaves, and a marked decrease in the quantum yield of PSII evaluated by chlorophyll fluorescence. In PsbP-deficient plant, most PSII core subunits were accumulated in thylakoids, whereas PsbQ, which requires PsbP to bind PSII in vitro, was dramatically decreased. PSII without PsbP was hypersensitive to light and rapidly inactivated when the repair process of the damaged PSII was inhibited by chloramphenicol. Furthermore, thermoluminescence studies showed that the catalytic manganese cluster in PsbP-deficient leaves was markedly unstable and readily disassembled in the dark. The present results demonstrated that PsbP, but not PsbQ, is indispensable for the normal PSII function in higher plants in vivo.</jats:p>

収録刊行物

  • Plant Physiology

    Plant Physiology 139 (3), 1175-1184, 2005-10-21

    Oxford University Press (OUP)

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