<i>GOLD HULL AND INTERNODE2</i> Encodes a Primarily Multifunctional Cinnamyl-Alcohol Dehydrogenase in Rice

• Kewei Zhang

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Qian Qian

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Zejun Huang

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Yiqin Wang

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Ming Li

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Lilan Hong

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Dali Zeng

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Minghong Gu

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Chengcai Chu

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

• Zhukuan Cheng

  State Key Laboratory of Plant Genomics and National Center for Plant Gene Research, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing 100101, People's Republic of China (K.Z., Z.H., Y.W., M.L., L.H., D.Z., C.C., Z.C.); State Key Laboratory of Rice Biology, China National Rice Research Institute, Hangzhou 310006, People's Republic of China (Q.Q., D.Z.); Departme

抄録

Lignin content and composition are two important agronomic traits for the utilization of agricultural residues. Rice (Oryza sativa) gold hull and internode phenotype is a classical morphological marker trait that has long been applied to breeding and genetics study. In this study, we have cloned the GOLD HULL AND INTERNODE2 (GH2) gene in rice using a map-based cloning approach. The result shows that the gh2 mutant is a lignin-deficient mutant, and GH2 encodes a cinnamyl-alcohol dehydrogenase (CAD). Consistent with this finding, extracts from roots, internodes, hulls, and panicles of the gh2 plants exhibited drastically reduced CAD activity and undetectable sinapyl alcohol dehydrogenase activity. When expressed in Escherichia coli, purified recombinant GH2 was found to exhibit strong catalytic ability toward coniferaldehyde and sinapaldehyde, while the mutant protein gh2 completely lost the corresponding CAD and sinapyl alcohol dehydrogenase activities. Further phenotypic analysis of the gh2 mutant plants revealed that the p-hydroxyphenyl, guaiacyl, and sinapyl monomers were reduced in almost the same ratio compared to the wild type. Our results suggest GH2 acts as a primarily multifunctional CAD to synthesize coniferyl and sinapyl alcohol precursors in rice lignin biosynthesis.

収録刊行物

• Plant Physiology

  Plant Physiology 140 (3), 972-983, 2006-01-27

  Oxford University Press (OUP)

キーワード

• Plant Science
• Genetics
• Physiology

詳細情報

• CRID: 1360011145112233472
• NII論文ID: 30019363419
• DOI: 10.1104/pp.105.073007
• ISSN: 15322548
• ISSN: 00320889
• Web Site: http://academic.oup.com/plphys/article-pdf/140/3/972/37865356/plphys_v140_3_972.pdf
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