Rat Insulin Genes: Construction of Plasmids Containing the Coding Sequences

• Axel Ullrich

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

• John Shine

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

• John Chirgwin

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

• Raymond Pictet

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

• Edmund Tischer

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

• William J. Rutter

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

• Howard M. Goodman

  Department of Biochemistry and Biophysics, University of California, San Francisco, 94143

抄録

Recombinant bacterial plasmids have been constructed that contain complementary DNA prepared from rat islets of Langerhans messenger RNA. Three plasmids contain cloned sequences representing the complete coding region of rat proinsulin I, part of the preproinsulin I prepeptide, and the untranslated 3′ terminal region of the mRNA. A fourth plasmid contains sequences derived from the A chain region of rat preproinsulin II.

収録刊行物

• Science

  Science 196 (4296), 1313-1319, 1977-06-17

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1360574096309015552
• NII論文ID: 30020510480
• DOI: 10.1126/science.325648
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.325648
• データソース種別:
  • Crossref
  • CiNii Articles