Expression of a Bacterial Gene in Mammalian Cells

DOI Web Site 被引用文献7件
  • R. C. Mulligan
    Department of Biochemistry, Stanford University Medical Center, Stanford, California 94305.
  • P. Berg
    Department of Biochemistry, Stanford University Medical Center, Stanford, California 94305.

抄録

<jats:p> Transfection of cultured monkey kidney cells with recombinant DNA constructed with a cloned <jats:italic>Escherichia coli</jats:italic> gene that codes for xanthine-guanine phosphoribosyltransferase and several different SV40 DNA-based vectors, results in the synthesis of readily measurable quantities of the bacterial enzyme. Moreover, the physiological defect in purine nucleotide synthesis characteristic of human Lesch-Nyhan cells can be overcome by the introduction of the bacterial gene into these cells. </jats:p>

収録刊行物

  • Science

    Science 209 (4463), 1422-1427, 1980-09-19

    American Association for the Advancement of Science (AAAS)

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