Monocyte Chemotaxis: Stimulation by Specific Exosite Region in Thrombin

Rachel Bar-Shavit, Arnold Kahn, George D. Wilner, John W. Fenton

doi:10.1126/science.6836310

Monocyte Chemotaxis: Stimulation by Specific Exosite Region in Thrombin

DOI Web Site 被引用文献7件

Rachel Bar-Shavit

Jewish Hospital and Departments of Pathology and Medicine and Division of Cell Biology, Washington University, St. Louis, Missouri 63130
Arnold Kahn

Jewish Hospital and Departments of Pathology and Medicine and Division of Cell Biology, Washington University, St. Louis, Missouri 63130
George D. Wilner

Jewish Hospital and Departments of Pathology and Medicine and Division of Cell Biology, Washington University, St. Louis, Missouri 63130
John W. Fenton

Center for Laboratories and Research, New York State Department of Health, Albany 12201

抄録

<jats:p>Human α-thrombin is a potent chemoattractant for human monocytes, with optimum activity occurring at about 10 nanomoles per liter. A variety of thrombins that were chemically modified to alter procoagulant or esterolytic functions showed a similar optimum activity, but complexes of prothrombin or α-thrombin with either antithrombin III or hirudin did not. These findings indicate that the regions in thrombin responsible for monocyte chemotaxis are proximate to those involved in certain protein recognition interactions of α-thrombin (for example, hirudin binding) but are distinct from the catalytic site and from certain exosites required for clotting.</jats:p>