Molecular Cloning of the Chromosomal Breakpoint of B-Cell Lymphomas and Leukemias with the t(11;14) Chromosome Translocation

• Yoshihide Tsujimoto

  Wistar Institute and the Graduate Group of Molecular Biology, University of Pennsylvania, Philadelphia 19104

• Jorge Yunis

  Wistar Institute and the Graduate Group of Molecular Biology, University of Pennsylvania, Philadelphia 19104

• Louise Onorato-Showe

  Department of Laboratory Medicine and Pathology and the Department of Medicine, University of Minnesota Medical School, Minneapolis 55455

• Jan Erikson

  Wistar Institute and the Graduate Group of Molecular Biology, University of Pennsylvania, Philadelphia 19104

• Peter C. Nowell

  Department of Pathology and Laboratory Medicine, University of Pennsylvania School of Medicine, Philadelphia 19104

• Carlo M. Croce

  Wistar Institute and the Graduate Group of Molecular Biology, University of Pennsylvania, Philadelphia 19104

抄録

The chromosomal breakpoint of chronic lymphocytic leukemia (CLL) cells of the B-cell type carrying the translocated long arms of chromosomes 11 and 14 [t(11;14) (q13;q32)] was cloned. The breakpoint was found to be within the joining segment of the human heavy chain locus on the translocated long arm of chromosome 14. A probe that is specific for chromosome 11 and that maps immediately 5′ to the breakpoint on the 14q + chromosome was isolated. The probe detected a rearrangement of the homologous genomic DNA segment in the parental CLL cells and also in DNA from a diffuse large cell lymphoma with the t(11;14) translocation. This rearranged DNA segment was not present in Burkitt lymphoma cells with the t(8;14) translocation or in nonneoplastic human lymphoblastoid cells. The probe can thus be used to identify and characterize a gene located on band q13 of chromosome 11 that appears to be involved in the malignant transformation of human B cells carrying the t(11;14) translocation. This gene, named bcl-1 , appears to be unrelated to any of the known retrovirus oncogenes described to date.

収録刊行物

• Science

  Science 224 (4656), 1403-1406, 1984-06-29

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1361418519541746944
• NII論文ID: 30020527298
• DOI: 10.1126/science.6610211
• ISSN: 10959203
• ISSN: 00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.6610211
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