Complementary DNA Sequencing: Expressed Sequence Tags and Human Genome Project

• Mark D. Adams

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Jenny M. Kelley

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Jeannine D. Gocayne

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Mark Dubnick

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Mihael H. Polymeropoulos

  Laboratory of Biochemical Genetics, National Institute of Mental Health, Neuroscience Center at St. Elizabeth's Hospital, Washington, DC 20032.

• Hong Xiao

  Laboratory of Biochemical Genetics, National Institute of Mental Health, Neuroscience Center at St. Elizabeth's Hospital, Washington, DC 20032.

• Carl R. Merril

  Laboratory of Biochemical Genetics, National Institute of Mental Health, Neuroscience Center at St. Elizabeth's Hospital, Washington, DC 20032.

• Andrew Wu

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Bjorn Olde

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Ruben F. Moreno

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• Anthony R. Kerlavage

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• W. Richard McCombie

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

• J. Craig Venter

  Section of Receptor Biochemistry and Molecular Biology, National Institute of Neurological Disorders and Stroke, National Institutes of Health, Bethesda, MD 20892.

抄録

Automated partial DNA sequencing was conducted on more than 600 randomly selected human brain complementary DNA (cDNA) clones to generate expressed sequence tags (ESTs). ESTs have applications in the discovery of new human genes, mapping of the human genome, and identification of coding regions in genomic sequences. Of the sequences generated, 337 represent new genes, including 48 with significant similarity to genes from other organisms, such as a yeast RNA polymerase II subunit; Drosophila kinesin, Notch , and Enhancer of split ; and a murine tyrosine kinase receptor. Forty-six ESTs were mapped to chromosomes after amplification by the polymerase chain reaction. This fast approach to cDNA characterization will facilitate the tagging of most human genes in a few years at a fraction of the cost of complete genomic sequencing, provide new genetic markers, and serve as a resource in diverse biological research fields.

収録刊行物

• Science

  Science 252 (5013), 1651-1656, 1991-06-21

  American Association for the Advancement of Science (AAAS)

キーワード

• Multidisciplinary

詳細情報

• CRID: 1364233268417965696
• NII論文ID: 30020540120
• DOI: 10.1126/science.2047873
• ISSN: 10959203
• ISSN: 00368075
• ISSN: http://id.crossref.org/issn/00368075
• Web Site: https://www.science.org/doi/pdf/10.1126/science.2047873
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