A Genetic Linkage Map for the Zebrafish

John H. Postlethwait, Stephen L. Johnson, Clare N. Midson, William S. Talbot, Michael Gates, Eric W. Ballinger, Dana Africa, Rebecca Andrews, Tim Carl, Judith S. Eisen, Sally Horne, Charles B. Kimmel, Mark Hutchinson, Michele Johnson, Andre Rodriguez

doi:10.1126/science.8171321

A Genetic Linkage Map for the Zebrafish

DOI Web Site 被引用文献14件

John H. Postlethwait

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Stephen L. Johnson

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Clare N. Midson

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
William S. Talbot

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Michael Gates

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Eric W. Ballinger

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Dana Africa

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Rebecca Andrews

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Tim Carl

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Judith S. Eisen

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Sally Horne

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Charles B. Kimmel

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Mark Hutchinson

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Michele Johnson

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.
Andre Rodriguez

Institute of Neurosciences, University of Oregon, Eugene, OR 97403, USA.

抄録

<jats:p> To facilitate molecular genetic analysis of vertebrate development, haploid genetics was used to construct a recombination map for the zebrafish <jats:italic>Danio</jats:italic> ( <jats:italic>Brachydanio</jats:italic> ) <jats:italic>rerio</jats:italic> . The map consists of 401 random amplified polymorphic DNAs (RAPDs) and 13 simple sequence repeats spaced at an average interval of 5.8 centimorgans. Strategies that exploit the advantages of haploid genetics and RAPD markers were developed that quickly mapped lethal and visible mutations and that placed cloned genes on the map. This map is useful for the position-based cloning of mutant genes, the characterization of chromosome rearrangements, and the investigation of evolution in vertebrate genomes. </jats:p>