Horizontal Transfer of <i>bla</i> _CMY -Bearing Plasmids among Clinical <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates and Emergence of Cefepime-Hydrolyzing CMY-19

• Jun-ichi Wachino

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

• Hiroshi Kurokawa

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

• Satowa Suzuki

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

• Kunikazu Yamane

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

• Naohiro Shibata

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

• Kouji Kimura

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

• Yasuyoshi Ike

  Department of Bacteriology and Bacterial Infection Control, Gunma University Graduate School of Medicine, Gunma, Japan

• Yoshichika Arakawa

  Department of Bacterial Pathogenesis and Infection Control, National Institute of Infectious Diseases, Tokyo

抄録

Nine Escherichia coli and 5 Klebsiella pneumoniae clinical isolates resistant to various cephalosporins and cephamycins were identified in a Japanese general hospital between 1995 and 1997. All nine E. coli isolates and one K. pneumoniae isolate carried bla CMY-9 , while the other four K. pneumoniae isolates harbored a variant of bla CMY-9 , namely, bla CMY-19 . The pulsed-field gel electrophoresis patterns of the nine CMY-9-producing E. coli isolates were almost identical, suggesting their clonal relatedness, while those of the five K. pneumoniae isolates were divergent. Plasmid profiles, Southern hybridization, and conjugation assays revealed that the genes for the CMY-9 and the CMY-19 β-lactamases were located on very similar conjugative plasmids in E. coli and K. pneumoniae . The genetic environment of bla CMY-19 was identical to that of bla CMY-9 . A single amino acid substitution, I292S, adjacent to the H-10 helix region was observed between CMY-9 and CMY-19. This substitution was suggested to be responsible for the expansion of the hydrolyzing activity against several broad-spectrum cephalosporins, and this finding was consistent with the kinetic parameters determined with purified enzymes. These findings suggest that the bla CMY-19 genes found in the four K. pneumoniae isolates might have originated from bla CMY-9 gene following a point mutation and dispersed among genetically different K. pneumoniae isolates via a large transferable plasmid.

収録刊行物

• Antimicrobial Agents and Chemotherapy

  Antimicrobial Agents and Chemotherapy 50 (2), 534-541, 2006-02

  American Society for Microbiology

キーワード

• Infectious Diseases
• Pharmacology (medical)
• Pharmacology

詳細情報

• CRID: 1360011144517297024
• NII論文ID: 30020633061
• DOI: 10.1128/aac.50.2.534-541.2006
• ISSN: 10986596
• ISSN: 00664804
• Web Site: https://journals.asm.org/doi/pdf/10.1128/AAC.50.2.534-541.2006
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