Human Parvovirus B19 Nonstructural Protein (NS1) Induces Cell Cycle Arrest at G <sub>1</sub> Phase
-
- Eiji Morita
- Department of Microbiology and Immunology, School of Medicine, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8575
-
- Akitoshi Nakashima
- Department of Microbiology and Immunology, School of Medicine, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8575
-
- Hironobu Asao
- Department of Microbiology and Immunology, School of Medicine, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8575
-
- Hiroyuki Sato
- Fukuoka Red Cross Blood Center, Fukuoka 818-8588, Japan
-
- Kazuo Sugamura
- Department of Microbiology and Immunology, School of Medicine, Tohoku University, Aoba-ku, Sendai, Miyagi 980-8575
抄録
<jats:title>ABSTRACT</jats:title> <jats:p> Human parvovirus B19 infects predominantly erythroid precursor cells, leading to inhibition of erythropoiesis. This erythroid cell damage is mediated by the viral nonstructural protein 1 (NS1) through an apoptotic mechanism. We previously demonstrated that B19 virus infection induces G <jats:sub>2</jats:sub> arrest in erythroid UT7/Epo-S1 cells; however, the role of NS1 in regulating cell cycle arrest is unknown. In this report, by using paclitaxel, a mitotic inhibitor, we show that B19 virus infection induces not only G <jats:sub>2</jats:sub> arrest but also G <jats:sub>1</jats:sub> arrest. Interestingly, UV-irradiated B19 virus, which has inactivated the expression of NS1, still harbors the ability to induce G <jats:sub>2</jats:sub> arrest but not G <jats:sub>1</jats:sub> arrest. Furthermore, treatment with caffeine, a G <jats:sub>2</jats:sub> checkpoint inhibitor, abrogated the B19 virus-induced G <jats:sub>2</jats:sub> arrest despite expression of NS1. These results suggest that the B19 virus-induced G <jats:sub>2</jats:sub> arrest is not mediated by NS1 expression. We also found that NS1-transfected UT7/Epo-S1 and 293T cells induced cell cycle arrest at the G <jats:sub>1</jats:sub> phase. These results indicate that NS1 expression plays a critical role in G <jats:sub>1</jats:sub> arrest induced by B19 virus. Furthermore, NS1 expression significantly increased p21/WAF1 expression, a cyclin-dependent kinase inhibitor that induces G <jats:sub>1</jats:sub> arrest. Thus, G <jats:sub>1</jats:sub> arrest mediated by NS1 may be a prerequisite for the apoptotic damage of erythroid progenitor cells upon B19 virus infection. </jats:p>
収録刊行物
-
- Journal of Virology
-
Journal of Virology 77 (5), 2915-2921, 2003-03
American Society for Microbiology
- Tweet
詳細情報 詳細情報について
-
- CRID
- 1360018298136976384
-
- NII論文ID
- 30020797799
-
- ISSN
- 10985514
- 0022538X
-
- データソース種別
-
- Crossref
- CiNii Articles