Critical Role of Epstein-Barr Virus (EBV)-Encoded RNA in Efficient EBV-Induced B-Lymphocyte Growth Transformation

• Misako Yajima

  Department of Tumor Virology

• Teru Kanda

  Center for Virus Vector Development, Institute for Genetic Medicine, Hokkaido University, Sapporo, Japan

• Kenzo Takada

  Department of Tumor Virology

抄録

It was demonstrated that Epstein-Barr virus (EBV)-encoded small RNAs (EBERs) were nonessential for B-lymphocyte growth transformation. We revisited this issue by producing a large quantity of EBER-deleted EBV by using an Akata cell system. Although the EBER-deleted virus efficiently infected B lymphocytes, its 50% transforming dose was approximately 100-fold less than that of the EBER-positive EBV. We then engineered the genome of EBER-deleted virus and generated a recombinant virus with the EBER genes reconstituted at their native locus. The resultant EBER-reconstituted EBV exhibited restored transforming ability. In addition, lymphoblastoid cell lines established with the EBER-deleted EBV grew significantly more slowly than those established with wild-type or EBER-reconstituted EBV, and the difference between the growth rates was especially highlighted when the cells were plated at low cell densities. These results clearly demonstrate that EBERs significantly contribute to the efficient growth transformation of B lymphocytes by enhancing the growth potential of transformed lymphocytes.

収録刊行物

• Journal of Virology

  Journal of Virology 79 (7), 4298-4307, 2005-04

  American Society for Microbiology

キーワード

• Virology
• Insect Science
• Immunology
• Microbiology

詳細情報

• CRID: 1360574093611018240
• NII論文ID: 30020802076
• DOI: 10.1128/jvi.79.7.4298-4307.2005
• ISSN: 10985514
• ISSN: 0022538X
• Web Site: https://journals.asm.org/doi/pdf/10.1128/JVI.79.7.4298-4307.2005
• データソース種別:
  • Crossref
  • CiNii Articles
  • KAKEN