Attenuated Nontoxinogenic and Nonencapsulated Recombinant <i>Bacillus anthracis</i> Spore Vaccines Protect against Anthrax

• S. Cohen

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• I. Mendelson

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• Z. Altboum

  <!--label omitted: 2-->Infectious Diseases,2 and

• D. Kobiler

  <!--label omitted: 2-->Infectious Diseases,2 and

• E. Elhanany

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• T. Bino

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• M. Leitner

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• I. Inbar

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• H. Rosenberg

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• Y. Gozes

  <!--label omitted: 2-->Infectious Diseases,2 and

• R. Barak

  <!--label omitted: 3-->Analytical Chemistry,3 Israel Institute for Biological Research, Ness-Ziona 74100, Israel

• M. Fisher

  <!--label omitted: 2-->Infectious Diseases,2 and

• C. Kronman

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• B. Velan

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• A. Shafferman

  <!--label omitted: 1-->Departments of Biochemistry and Molecular Genetics,1

• D. L. Burns

  editor

抄録

Several highly attenuated spore-forming nontoxinogenic and nonencapsulated Bacillus anthracis vaccines differing in levels of expression of recombinant protective antigen (rPA) were constructed. Biochemical analyses (including electrospray mass spectroscopy and N terminus amino acid sequencing) as well as biological and immunological tests demonstrated that the rPA retains the characteristics of native PA. A single immunization of guinea pigs with 5 × 10 7 spores of one of these recombinant strains, MASC-10, expressing high levels of rPA (≥100 μg/ml) from a constitutive heterologous promoter induced high titers of neutralizing anti-PA antibodies. This immune response was long lasting (at least 12 months) and provided protection against a lethal challenge of virulent (Vollum) anthrax spores. The recombinant B. anthracis spore vaccine appears to be more efficacious than the vegetative cell vaccine. Furthermore, while results clearly suggest a direct correlation between the level of expression of PA and the potency of the vaccine, they also suggest that some B. anthracis spore-associated antigen(s) may contribute in a significant manner to protective immunity.

収録刊行物

• Infection and Immunity

  Infection and Immunity 68 (8), 4549-4558, 2000-08

  American Society for Microbiology

キーワード

• Infectious Diseases
• Immunology
• Microbiology
• Parasitology

詳細情報

• CRID: 1360011144118395904
• NII論文ID: 30020829857
• NII書誌ID: AA00673732
• DOI: 10.1128/iai.68.8.4549-4558.2000
• ISSN: 10985522
• ISSN: 00199567
• Web Site: https://journals.asm.org/doi/pdf/10.1128/IAI.68.8.4549-4558.2000
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